Micro RNA-30b (inhibitor) nanoparticles suppressed the lipopolysaccharide (LPS)-induced acute kidney injury.

Abstract

The main aim of present study is to evaluate the effect of miR-30b on the function of human proximal tubular epithelial cell line HK-2 cells. For this purpose, miRNA was loaded in an ionically cross-linked polysaccharide nanoparticle. The authors have demonstrated the influence of miR-30b mimic and inhibitor in HK-2 cell killing effect. Lipopolysaccharide (LPS) significantly increased the level of inflammatory cytokines of TNF-α, IL-1β and level was further increased with the treatment of PAg-miR mimic consistent with the cell viability assay. Interestingly, PAg-miR inhibitor significantly downregulated the expression of inflammatory cytokines and thereby reduced the inflammation in the body. Western blot analysis showed that LPS induced severe apoptosis of HK-2 cells and the apoptosis was further promoted by the PAg-miR (mimic). In contrast, PAg-miR (inhibitor) alleviated the apoptosis of HK-2 cells as indicated in the significantly reduced levels of Bax and c-Caspase-3 proteins. Overall, miR-30b promoted LPS-induced HK-2 cell inflammatory injury by inducing the apoptosis and by releasing inflammatory cytokines, as well as by impairing autophagy process.