Abstract
Mid-Ultraviolet (UVB) has a significant influence on human health. In this study, human erythrocytes were exposed to UVB to investigate the effects of UVB radiation on erythrocytes membrane. And Micro-Raman spectroscopy was employed to detect the damage. Principal component analysis (PCA) was used to classify the control erythrocytes and the irradiated erythrocytes. Results showed that the erythrocytes membrane was damaged by Mid-Ultraviolet (UVB) radiation. The intensity of the Raman peaks at 1126cm−1 and 1082cm−1 were used to calculate the Longitudinal Order-Parameters in Chains (Strans) which can present the liquidity and ionic permeability of erythrocyte membrane. After UVB radiation for 30min, both the liquidity and ionic permeability decreased. At the same time, the intensity of the peaks at 1302cm−1 (α-helix), 1254cm−1 (random coil), 1452cm−1 and 1430cm−1 (CH2/CH3 stretch) have also changed which indicated the membrane protein also been damaged by UVB. In the whole process of radiation, the more UVB radiation dose the more damage on the erythrocyte membrane.
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