Micro-Raman spectroscopy for monitoring changes in periodontal ligaments and gingival crevicular fluid.

Carlo Camerlingo,Fabrizia D'Apuzzo,Letizia Perillo,Maria Lepore,Vincenzo Grassia

doi:10.3390/s141222552

Abstract

Micro-Raman Spectroscopy is an efficient method for analyzing biological specimens due to its sensitivity to subtle chemical and structural changes. The aim of this study was to use micro-Raman spectroscopy to analyze chemical and structural changes in periodontal ligament after orthodontic force application and in gingival crevicular fluid in presence of periodontal disease. The biopsy of periodontal ligament samples of premolars extracted for orthodontic reasons and the gingival crevicular fluid samples collected by using absorbent paper cones; were analyzed by micro-Raman spectroscopy. Changes of the secondary protein structure related to different times of orthodontic force application were reported; whereas an increase of carotene was revealed in patients affected by periodontal inflammation.

Highlights

The periodontal ligament (PDL) is a membrane-like connective tissue interposed between the tooth root and the alveolar bone of which the main component is represented by collagen fibers
Our preliminary study, based on biopsy of PDL samples of premolars extracted for orthodontic reasons and analyzed by μ-RS, confirmed its efficacy in evaluate, at a nanometer scale, the macromolecular folding of periodontal fibers after orthodontic force application [6]
Showed the secondary protein structure changes related to different times of orthodontic force application, which may be similar in presence of periodontal diseases

Summary

Introduction

The periodontal ligament (PDL) is a membrane-like connective tissue interposed between the tooth root and the alveolar bone of which the main component is represented by collagen fibers. This tissue has an important role in supporting the tooth in the bone socket of the jaw and in maintaining homeostasis of the surrounding tissues, such as alveolar bone and cementum [1]. Μ-RS showed the secondary protein structure changes related to different times of orthodontic force application, which may be similar in presence of periodontal diseases. Ngo et al [10]

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