Abstract
We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals. We show that the integration of microlenses and mirror surfaces in these devices results in an 8-fold increase in the fluorescence signal and in improved spatial resolution. Using an array of microlenses, massively parallel detection of droplets containing fluorescent dyes was achieved, leading to detection throughputs of about 2000 droplets per second and per lens, parallelized over 625 measurement points.
Highlights
We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals
We show that the integration of microlenses and mirror surfaces in these devices results in an 8-fold increase in the fluorescence signal and in improved spatial resolution
These conventional detection systems have limitations when applied for massively parallel detection: (i) optical systems with a high numerical aperture such as photomultipliers can offer sufficient sensitivity, but a multipoint measurement is difficult to achieve; (ii) small numerical aMax Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077
Summary
Micro-optical lens array for fluorescence detection in droplet-based microfluidics[3] We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals.
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