Micro-method for fluorimetric assay of retinol-binding protein in blood plasma

Abstract

1. 1. Holo retinol-binding protein was assayed by irradiating the pherogram of the plasma with ultraviolet light and measuring its yellow green fluorescence in the α 2 -globulin zone. 2. 2. The level of holo retinol-binding protein in normal adults was found to be 47.5 (± 1.3 S.E.) μg/ml. 3. 3. The mean level of total retinol-binding protein in one group of normal patients was 48.3 μg/ml compared to 42.3 μg/ml for the holo protein indicating that some apoprotein is usually present in human plasma.