Abstract
It is not generally recognized among physicians that the Kahn test can be performed with a drop or even half a drop of serum with a high degree of accuracy. In isolated cases, especially when drawing blood from infants, it is often difficult to obtain 1 cc., the minimum amount required for a regular three-tube Kahn test. In such cases, this laboratory, during the last several years, has been reporting to physicians the results of a one-tube test, utilizing the amount of serum available. One of the basic requirements of the Kahn reaction is the use of correct proportions of serum and antigen.<sup>1</sup>When this is adhered to, any measurable amounts of serum and antigen can be utilized in performing a Kahn test. The following plan is being carried out when sufficient serum (0.45 cc.) is not available for a complete three-tube Kahn test:
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