Abstract
Abstract Increased numbers and length of roots were produced from stem cuttings of ‘Crimson Pigmy’ barberry (Berberis thunbergii atropurpurea) treated with 3000 ppm IBA (50% ethanol/water, v/v). Two node explants from greenhouse-grown stock plants performed best if oriented vertically on woody plant medium with 5 or 10 μm BA and μm GA3. Up to 20 axillary shoots were produced from each explant in 3 months, and leaves that normally become spines grew in vitro as round green lamina with petioles. IBA did not stimulate rooting of microshoots, which are more difficult to root than cuttings. Chemical names used: 1H-indole-3-butanoic acid (IBA), N-(phenylmethyl)-1H-purin-6-amine (BA), and gibberellic acid (GA3).
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