Abstract
A method is discussed for the quantitative determination of prostaglandins and ecdysteroids in insects by pre-column derivatization and high-performance liquid chromatography. Prostaglandin and ecdysteroid fractions are first purified from homogenized insect organs by a Sep-Pak C 18 cartridge and derivatized quantitatively by treatment with 1-anthroyl nitrite. These derivatives are then used for quantitative high-performance liquid chromatography analysis on a C 18 column equipped with fluorescence detection. The detection limit is presently below 10 pg as an injected amount of the standard derivatives. The foregoing methods are applied for the determination of the prostaglandin and ecdysteroid titers in the various organs of an individual insect.
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