Abstract
La necesidad de contar con organismos nativos con potencial para el control biológico de plagas para el nogal pecanero, motivaron el registro de la micobiota y búsqueda de entomopatógenos en larvas del gusano barrenador del ruezno (GBR) Cydia caryana. Para ello, en el año 2013, en una huerta de nogal en La Laguna México, se recolectaron y procesaron 5 000 rueznos para extraer larvas de GBR, que fueron incubadas en cámara húmeda o suelo. De larvas obtenidas antes del 12 de septiembre y colocadas en suelo se recobró 9% de Fusarium spp. + Gliocladium spp., en contraste, ambos hongos aparecieron 40% en larvas obtenidas después de esa fecha. En larvas obtenidas en diciembre y se colocaron en cámara húmeda, Fusarium + Gliocladium spp. aparecieron 80%. Únicamente fue evidentemente a Metarhizium anisopliae como entomopatógenos de 5% de larvas del GBR colocadas en suelo después del 12 de septiembre, éste hongo también fue entomopatógenos del ácaro rojo gigante Trombidium sp., pulgón negro del nogal Melanocallis caryaefoliae, Garrapata Rhipicephalus sanguineus, y hormiga roja Solenopsis sp. Larvas de GBR pueden usarse como trampa para recobrar Metarhizium y probablemente otros hongos entomopatógenos en suelo de huertas de nogal pecanero. 
 
Highlights
Mexico, is considered a mega diverse country, including a source of natural enemies for pests and plant diseases, which are part of biological control programs in the world, our country, brings to the world more organisms for the biological control of pests than those he receives; only four genera of fungi and one of bacteria are widely used as biological control of pests
The objectives of the work were: i) to record the mycobiota and to find entomopathogenic fungi in GBR larvae inside the rinds and in larvae used as a trap in Micobiota y Metarhizium asilados de larvas del gusano barrenador del ruezno (Cydia caryana) al., 2011; Rodrígues et al, 2005; Sookar et al, 2008)
Three dates of harvesting of rinds were made, in the first two were collected nuts to obtain their rinds with signs of entry by the GBR, in the third date were collected 100 kg of rinds newly separated from the nut by means of industrial machinery.All the rinds obtained were stored in a cold room at temperature 5-8 °C until extracted their larvae and pupae of GBR
Summary
Mexico, is considered a mega diverse country, including a source of natural enemies for pests and plant diseases, which are part of biological control programs in the world, our country, brings to the world more organisms for the biological control of pests than those he receives; only four genera of fungi and one of bacteria are widely used as biological control of pests. In addition to the above, there is a need to protect against new pests in Mexico and other countries, so it is important to have native organisms with potential for biological pest control (Williams et al, 2013). Pests of importance to the walnut nut Carya illinoinensis (Wangenh.) K.Koch, such as nut borers (GBR) and rinds (GBN) Acrobasis nuxvorella (Neunzig) and Cydia caryana (Fitch) respectively (Nava and Ramírez, 2002), little is known of the fungi as natural enemies of both pests. In agricultural soils cultivated with sugarcane Saccharum officinarum L., bean Phaseolus vulgaris L. and maize Zea mays L., entomopathogenic fungi have been isolated (Barajas et al, 2009; Bautista et al, 2012; Hernández et al, 2011)
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