Abstract
Abstract This article describes various approaches to the analysis of quantitative responses assumed to follow a hyperbolic dose–response relationship (the Michaelis–Menten equation) characterized by the values of two parameters: the Michaelis constant (the dose at half‐maximal response) and the asymptotic maximum response. Methods include diagnostic graphical representations, including the double reciprocal or Lineweaver–Burk plot, the Hanes plot and the Eadie–Hofstee plot. Curve‐fitting and parameter estimation is described using either least squares or maximum likelihood. The latter includes using a generalized linear model incorporating a reciprocal link function and the possibility of nonnormally distributed errors. Robust and distribution‐free estimation procedures, such Theil's method (known as the direct linear plot in biochemistry) are also covered.
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