Micelle Based Spectrofluorimetric Determination of Chloroquine Phosphate in Commercial Formulation and Human Plasma

Abstract

Abstract Simple, sensitive and economic spectrofluorimetric method has been described for the determination of chloroquine phosphate in pure form, pharmaceutical formulations and spiked human plasma. In the developed method, fluorescence intensity of chloroquine phosphate was enhanced with 0.4 M sodium dodecyl sulfate in basic media of pH 10 at room temperature. The excitation and emission wavelengths of the fluorescent chloroquine phosphate are 330 nm and 369 nm respectively. The developed approach has a broad linear range (0.03–5 μg mL−1) with a correlation coefficient of 0.9976. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 3.38 × 10−3 μg mL−1 and 1.12 × 10−2 μg mL−1 respectively. The common additives and co-administered medications were investigated for their interferences effect in the assay. The method was validated statistically through recovery studies and successfully applied to chloroquine phosphate determination in bulk powder, pharmaceutical preparation and spiked human plasma samples. The percent recoveries were found to be in the range of 99.42–100.46 % for bulk powder, 97.48–101.21 % for pharmaceutical formulations and 96.49–98.48 % for spiked human plasma.