Abstract
A procedure for the separation of charged and neutral solutes in acidic micellar running buffers has been developed. The procedure is based on a zwitterionic surfactant 3-N,N-dimethylmyristylammoniopropanesulfonate (MAPS) and alkylsulfonates added to the running buffer at pH 4.0. The alkylsulfonates increase the migration-time window for elution of neutral substances by increasing electro-osmotic mobility and by creating a negative micellar electrophoretic mobility. The test solution contained opiates and adulterants found in heroin seizures. Both the test solution and actual heroin seizures were successfully separated using a running buffer containing 50 m M 6-aminocaproic acid adjusted to pH 4.0 with 50 m M MAPS, 5 m M 1-heptanesulfonic acid and 10% acetonitrile. The procedure offers an alternative to micellar electrokinetic chromatographic separations based on charged surfactants in alkaline buffers.
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