MIC, MBIC, MBEC Analyses of Garlic Extract (Allium sativum) from Indonesian Variety Against Streptococcus mutans

Abstract

Garlic (Allium sativum) has been used for traditional medicine since ages. It has many uses such as an antioxidant, antiseptic, anti-viral, anti-fungal, anti-cancer, anti-aging, reduce the risk of heart disease, and antibacterial1. Aim: This study was designed to evaluate the inhibition and eradication ability for garlic against Streptococcus mutans, the bacteria that cause dental caries. Methods: Garlic extract was obtained from Ciwidey garlic maseration process in etanol 96%. MIC was a test to measure the inhibition ability of garlic extract against Streptococcus mutans in planktonic form, and MBIC in biofilms form. MBEC was a test to measure the eradication ability of garlic extract against Streptococcus mutans in biofilms form. Statistic analysis using ANOVA followed by post hoc with  ρ value <0.05. Results: The garlic extract showed MIC value at 9.39 % and MBIC value at 37.5 % but was not observed to have eradication activity against Streptococcus mutans in biofilms form up to concentration 37.5%. Conclusion: Garlic extract inhibit Streptococcus mutans both in planktonic and biofilm form. Garlic extract has no eradication activity against Streptococcus mutans in biofilms form up to concentration 37.5%.