MIC and NKG2D genes polymorphism in Mexican population

Abstract

Event Abstract Back to Event MIC and NKG2D genes polymorphism in Mexican population Miriam Nieves-Ramirez1, Eyerahi Bravo-Flores1, Pedro Alegre-Crespo2, Maria Del Carmen Tapia-Lugo2 and Martha Perez-Rodriguez1* 1 Mexican Institute of Social Security, Pediatric Hospital, National Medical Center S-XXI, Mexico 2 Mexican Institute of Social Security, General Hospital 1A "Venados", Mexico Nowadays, different knowledge polymorphism in genes involved in the immune response helps to identify the susceptibility or risk in the development of different human diseases. Therefore, the objective was to determine the distribution of MIC gene polymorphisms and Single Nucleotide Polymorphisms (SNP) rs1049174, rs2255336 and rs2617160 of KLRK1 (NKG2D) gene in the Mexican population by their interaction, MICA/MICB-NKG2D. The polymorphism of MICA and MICB genes and the SNPs of NKG2D were analyzed by Reference Strand Conformation Analysis (RSCA) and Sequence Specific Oligonucleotide Probes (SSOP)-Single Specific Primer (SSP) and allelic discrimination, respectively. We genotyped 336 samples of persons clinically healthy and MICA, MICB and NKG2D polymorphisms were identified by RSCA, SSOP-SSP and allelic discrimination, techniques, respectively. The most frequent allele were MICA *002:01 (33.28%), MICB *005:02 (63.09%), NKG2D rs1049174 *T (61.31%), NKG2D rs2255336 *C (61.31%) and NKG2D rs2617160 *G (93.30%). We also found that the most frequent combination of genotypes in the genes MICA/MICB was *002:01-*002:01/*005:02-*005:02 with 7.44%, while the combination of genotypes at SNPs NKG2D in positions rs1049174/rs2255336/rs2617160 was *A-*T/*C-*G/*G-*G with 45.53%. The data obtained provide other genetic markers involved in the immune innate response that can be considered for the study of susceptibility or protection in different diseases of public health and participation in transplants in Mexican individuals. Acknowledgements Work performed with support FOFOI 2005/1/I/016, CONACYT 14019. Keywords: Polymorphism, Genetic, Polymorphism, Single Nucleotide, Polymorphism, Single-Stranded Conformational, Sequence Specific Oligonucleotide Probes, Single Specific Primer Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Nieves-Ramirez M, Bravo-Flores E, Alegre-Crespo P, Tapia-Lugo M and Perez-Rodriguez M (2013). MIC and NKG2D genes polymorphism in Mexican population. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00764 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Martha Perez-Rodriguez, Mexican Institute of Social Security, Pediatric Hospital, National Medical Center S-XXI, Mexico City, Mexico, meperezrodriguez@yahoo.com.mx Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Miriam Nieves-Ramirez Eyerahi Bravo-Flores Pedro Alegre-Crespo Maria Del Carmen Tapia-Lugo Martha Perez-Rodriguez Google Miriam Nieves-Ramirez Eyerahi Bravo-Flores Pedro Alegre-Crespo Maria Del Carmen Tapia-Lugo Martha Perez-Rodriguez Google Scholar Miriam Nieves-Ramirez Eyerahi Bravo-Flores Pedro Alegre-Crespo Maria Del Carmen Tapia-Lugo Martha Perez-Rodriguez PubMed Miriam Nieves-Ramirez Eyerahi Bravo-Flores Pedro Alegre-Crespo Maria Del Carmen Tapia-Lugo Martha Perez-Rodriguez Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.