MhYTP1 and MhYTP2 from Apple Confer Tolerance to Multiple Abiotic Stresses in Arabidopsis thaliana

Na Wang,Fengwang Ma,Bowen Liang,Xin Jia,Tianli Guo,Xiaoqing Gong,Xun Sun,Yun Shao,Ping Wang

doi:10.3389/fpls.2017.01367

Na Wang, Fengwang Ma + Show 7 more

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https://doi.org/10.3389/fpls.2017.01367

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Abstract

The first YTH domain-containing RNA binding protein (YTP) was found in rat, where it was related to oxidative stress. Unlike characterizations in yeast and animals, functions of plant YTPs are less clear. Malus hupehensis (Pamp.) Rehd. YTP1 and YTP2 (MhYTP1 and MhYTP2) are known to be active in leaf senescence and fruit ripening. However, no research has been published about their roles in stress responses. Here, we investigate the stress-related functions of MhYTP1 and MhYTP2 in Arabidopsis thaliana. Both of the two genes participated in salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA) signaling and play roles in plant responses to oxidative stress, chilling, high temperature, high salinity, and mannitol induced physiological drought stress. Moreover, MhYTP1 plays leading roles in SA and ABA signaling, and MhYTP2 plays leading roles in JA signaling and oxidative stress responses. These results will fill a gap in our knowledge about plant YTPs and stress responses and provide a foundation for future attempts to improve stress tolerance in apple.

Highlights

Phytohormones such as, abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA) play vital roles in stress responses (Reje et al, 2014)
We noted that GUS expression was induced by methyl jasmonate (MeJA) treatment, especially in plants transformed with the Malus hupehensis YTP2 (MhYTP2) promoter region (Figure 2A)
The TCA-Element occurs in the Malus hupehensis YTP1 (MhYTP1) promoter region but not in the MhYTP2 promoter

Summary

Introduction

Phytohormones such as, abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA) play vital roles in stress responses (Reje et al, 2014). ABA is active when plants are exposed to heavy metals, drought, extremes in temperature, high salinity, or radiation It functions in various developmental processes, including seed germination and dormancy, and the closure of stomata (Vishwakarma et al, 2017). Both JA and SA are involved in responses to biotic stresses. The JA-signaling pathway is critical to the development of resistance to necrotrophic pathogens that derive nutrients from dead or dying cells, while the SA-signaling pathway is mainly activated against biotrophic pathogens that obtain nutrients from living host tissues (Glazebrook, 2005). In plants resistant to pathogen infections, the JA- and SA-defense pathways generally interact antagonistically (Verhage et al, 2010)

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