胃癌组织中MGMT、RASSF1A基因启动子区甲基化研究

Abstract

目的：探讨MGMT、RASSF1A启动子甲基化状况在胃癌发生、发展中的作用。方法：用甲基特异性聚合酶扩增链式反应(methylation specific PCR, MSP)检测60例胃癌组织及相应癌旁正常组织MGMT、RASSF1A基因启动子的甲基化状况，用实时荧光定量逆转录–多聚合酶链反应(real-time RT-PCR)和免疫组织化学SP法分别检测基因MGMT、RASSF1A的mRNA和蛋白的表达情况。结果：胃癌组织中MGMT、RASSF1A基因的甲基化阳性率较正常组织明显升高(P Objective: To investigate the functions of hypermethylation of MGMT and RASSF1A genes in human gastric cancer. Methods: Methylation status of the MGMT and RASSF1Agenes in 60 gastric carcinoma tissues and 60 paired surgical marginal normal gastric tissues were detected using methylation-specific PCR. Real-time RT-PCR was used to detect the expression of MGMT and RASSF1A, and immunohistochemistry was used to detect the expression of MGMT and RASSF1Aproteins in the above samples. Results: The positive rates of promoter methylation of the MGMT and RASSF1Agenes were significantly higher in gastric cancer than those in normal tissue (both P .