Mg–Zn–Mn alloy extract induces the angiogenesis of human umbilical vein endothelial cells via FGF/FGFR signaling pathway

Abstract

Magnesium (Mg) and its alloys as a type of different biodegradable materials have been used in the musculoskeletal field because of their excellent biocompatibility, biodegradability and mechanical properties similar to bone; besides, Mg could promote osteoblast differentiation in vitro and induce the formation of new bone in vivo. In the present study, we prepared the extracts of Mg–Zn–Mn alloy and examined their effects on the angiogenesis of human umbilical vein endothelial cells (HUVECs). In the present study, we prepared Mg–Zn–Mn alloy extracts of different concentrations and cultured HUVECs with these extracts. The DNA synthesis capacity, the cell viability, and the tube formation capacity of HUVECs could be significantly induced by 6.25% Mg alloy extract. In the meantime, the ratios of p-FGFR/FGFR, p-PI3K/PI3K, and p-AKT/AKT were significantly increased by 6.25% Mg alloy extract treatment, while decreased by FGFR/FGFR signaling pathway inhibitor BFJ398, indicating that 6.25% Mg alloy extract could promote the angiogenesis of HUVECs via activating FGF/FGFR signaling pathway. In conclusion, these data indicate that 6.25% Mg–Zn–Mn alloy extract induces the angiogenesis of HUVECs via FGF signaling pathway. Further in vivo experiments are needed to further confirm the present in vitro findings.