Mg-Dependent Ecto-ATPase Activity inLeishmania tropica

Abstract

ATPase activity has been located on the external surface ofLeishmania tropica.SinceLeishmaniais known to have an ecto-acid phosphatase, in order to discard the possibility that the ATP hydrolysis observed was due to the acid phosphatase activity, the effect of pH in both activities was examined. In the pH range from 6.8 to 8.4, in which the cells were viable, the phosphatase activity decreased, while the ecto-ATPase activity increased. To confirm that the observed ATP hydrolysis was promoted by neither phosphatase nor 5′-nucleotidase activities, a few inhibitors for these enzymes were tested. Vanadate and NaF strongly inhibited the phosphatase activity; however, no effect was observed on ATPase activity. Neither levamizole nor tetramizole, two specific inhibitors of alkaline phosphatases, inhibited this activity. The lack of response to ammonium molybdate indicated that 5′-nucleotidase did not contribute to the ATP hydrolysis. Also, the lack of inhibition of the ATP hydrolysis by high concentrations of ADP at nonsaturating concentrations of ATP discarded the possibility of any ATP diphosphohydrolase activity. The ATPase here described was stimulated by MgCl2but not by CaCl2. In the absence of divalent metal, a low level of ATP hydrolysis was observed, and CaCl2varying from 0.1 to 10 mmdid not increase the ATPase activity. At 5 mmATP, half-maximal stimulation of ATP hydrolysis was obtained with 0.29 ± 0.02 mmMgCl2. The apparentKmfor Mg-ATP2−was 0.13 ± 0.01 mmand free Mg2+did not increase the ATPase activity. ATP was the best substrate for this enzyme. Other nucleotides such as ITP, CTP, GTP, UTP, and ADP produced lower reaction rates. To confirm that this Mg-dependent ATPase was an ecto-ATPase, an impermeant inhibitor, 4,4′-diisothiocyanostylbene-2,2′-disulfonic acid was used. This amino/sulfhydryl-reactive reagent did inhibit the Mg-ecto-ATPase activity in a dose-dependent manner (I0.5= 27.5 ± 1.8 μm).