Abstract
Oxygen affinities of metronidazole susceptible and resistant isolates of the parasitic flagellate protozoon Trichomonas vaginalis were determined by mass spectrometric methods. Apparent O 2 K m values for the respiration of non-proliferating cell suspensions were about 10-fold higher for metronidazole resistant strains than for the susceptible strains C1-NIH or NYH-286. Simultaneous monitoring of hydrogen evolution in the presence of increasing O 2 tensions enabled apparent K i values for H 2 to be determined; and this function was independent of metronidazole susceptibility. Apparent O 2 affinities of the hydrogenosomal and non-sedimentable fractions were determined for the strains CDC 85 (metronidazole resistant) and C1-NIH, which showed the deficiency in the O 2 scavenging capacity by the resistant strain to be associated with the hydrogenosome-containing fraction.
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