Abstract
Three-dimensional quantitative phase imaging (3D QPI) delivers volumetric information about the refractive index (RI) distribution within microobjects and offers new solutions to outstanding challenges in cell biology and pathology [1-3]. Such imaging and measurement capabilities are of great interest to study the biophysical properties of biological microstructures such as single cells, cell aggregates, tissues or even small organisms, especially as they are complemented by high spatial resolution and label free sample preparation. Therefore, along with the development of multiplicity of research 3D QPI systems, the commercial devices using either spatial light interference (Phi Optics, Inc) or holographic tomography (TomoCube, Inc and NanoLive, Ltd) entered the market, readily tailored for microbiological applications. Despite of commercialization of these 3D QPI systems, their performance have not been fully quantified experimentally.
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