Abstract
Sepsis is a systemic inflammatory response syndrome (SIRS) caused by infection, with complicated pathological mechanism and extremely high prevalence and fatality rate. In this study, we investigated the role of METRNL in sepsis-induced renal injury and to identify potential downstream molecules. Male C57BL/6 mice were subjected to laparotomy followed by extracorporeal cecum mobilization and ligation as vivo model. THP-1 cells were stimulated with LPS as vitro model. As compared to the normal tissue or normal serum samples, the tissue and serum of METRNL expression levels in sepsis-induced renal injury were reduced. METRNL protein reduced inflammation and inhibited renal injury in sepsis mice model. METRNL up-regulation inhibited inflammation in vitro model. The inhibition of METRNL promoted inflammation and renal injury in sepsis mice model. METRNL reduced inflammation in sepsis-induced renal injury via PPARδ-dependent pathways. In conclusion, we are the first to identify METRNL as a co-activator of PPARδ to inhibit inflammation in sepsis-induced renal injury and potentiate the activity target of renal injury.
Highlights
Sepsis is a systemic inflammatory response syndrome (SIRS) caused by infection, with complicated pathological mechanism and extremely high prevalence and fatality rate (Nadeem et al, 2021; Ma et al, 2019)
To observe the function of METRNL on inflammation in sepsis-induced renal injury, we used reorganized METRNL protein to inject into mice with sepsis
In order to verify the effects of METRNL on inflammation in vitro model, METRNL plasmid induced METRNL expression, reduced TNF-α, IL-1β, IL-6 and IL-4 levels, and increased IL10 levels in vitro model of sepsis (Figure 4A-F)
Summary
Sepsis is a systemic inflammatory response syndrome (SIRS) caused by infection, with complicated pathological mechanism and extremely high prevalence and fatality rate (Nadeem et al, 2021; Ma et al, 2019). Sepsis is easy to develop into septic shock and multiple organ dysfunction syndrome (MODS), which is life-threatening (Nadeem et al, 2021; Ma et al, 2019). Acute kidney injury (AKI) pathologically characterized by progressively declined renal function is a common complication of sepsis (Wang et al, 2021). Epidemiological studies have shown that approximately 40% of patients with sepsis will develop AKI (Yoo et al, 2020). A lack of effective prognostic evaluation indicators is one of the main causes for the high mortality rate of AKI (Liu et al, 2020)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
