Metopus, Cyclidium and Sonderia: ciliates enriched and cultured from sulfureta of a microbial mat community

Abstract

Metopus contortus (Class Spirotrichea), Cyclidium borrori, Cyclidium sp., Sonderia tubigula, and two other species of Sonderia (Class Oligohymenophorea) were enriched from the sulfureta of microbial mat communities at Laguna Figueroa near San Quintin, Baja California, Mexico. The ability of some of these species to grow under anaerobic, high-sulfide conditions was demonstrated. The ultrastructural morphology of Metopus and Cyclidium revealed adaptations to anaerobic, high sulfide niches. Metopus lacks typical mitochondria and has structures which may be intracellular bacterial symbionts. Cyclidium borrori, when grown aerobically, has mitochondria with tubular cristae. When it is grown anaerobically in the presence of high amounts of hydrogen sulfide the number of mitochondria remains the same but the mitochondria dedifferentiate, becoming smaller with less well-defined cristae.