Abstract
The objective of this study was to determine the most efficient method for the evaluation of the physiological quality of cowpea (V. unguiculata) seeds by the tetrazolium test. We evaluated two seed lots of two cowpea cultivars, BRS Guariba and BRS Novaera. The tetrazolium test was performed for each batch of seeds and cultivating, which underwent tetrazolium solution at concentrations of 0.075%, 0.25% and 0.5% for 3-4 h in BOD incubator at 35°C in absence of light. The seeds were evaluated for uniformity and intensity of color presented by the tissues, being classified as: weak staining, suitable staining or strong staining. To evaluate the efficiency of the tetrazolium test, seeds of both cultivars and their lots were submitted to the germination and vigor tests. The mean values found in the tests were compared by Tukey test (p <0.05) and calculated the simple correlation coefficients of Pearson (r). It was observed that the tetrazolium is effective to classify the force of cowpea seeds under subsequent immersion in tetrazolium solution at a concentration of 0.075%, for a period 4 h at 35°C.
Highlights
We evaluated two seed lots of two cowpea cultivars, BRS Guariba and BRS Novaera
The tetrazolium test was performed for each batch of seeds and cultivating, which underwent tetrazolium solution at concentrations of 0.075%, 0.25% and 0.5% for [3,4] h in BOD incubator at 35°C in absence of light
The seeds were evaluated for uniformity and intensity of color presented by the tissues, being classified as: weak staining, suitable staining or strong staining
Summary
Glauce Portela De Oliveira1*, Elisangela Clarete Camili e Otoniel Magalhães Morais. RESUMO O objetivo foi determinar o método de coloração, quanto ao tempo e concentração da solução, mais eficiente para avaliação da qualidade fisiológica de sementes de feijãocaupi (V. unguiculata) pelo teste de tetrazólio. O teste de tetrazólio foi realizado para sementes de cada lote e cultivar, as quais foram submetidas à solução de tetrazólio nas concentrações de 0,075%, 0,25% e 0,5% por 3 e 4 h, em incubadora tipo BOD a 35°C, na ausência de luz. Para avaliar a eficiência do teste de tetrazólio, as sementes das duas cultivares e os respectivos lotes foram submetidos aos testes de germinação e vigor. Observou-se que o teste de tetrazólio é eficiente para a classificação do vigor de sementes de feijão-caupi e posterior imersão em solução de tetrazólio na concentração de 0,075%, por um período de 4 h a 35oC.
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