Methylumbelliferyl- N-acetylneuraminic acid sialidase in human liver

Abstract

Human liver sialidase was measured using methylumbelliferyl- N-acetylneuraminic acid as a substrate. The enzyme activity was linear for only 20 min and linearity was not improved by adding albumin, CaCl 2, dithiothreitol, or Ep-459. The optimal pH was 4.5 and the apparent K m value, approximately 0.090 m m. Without substrate addition, the enzyme was unstable at temperatures between 0 and 37°C, retaining only 35 and 5% of its activity, respectively, after 8 1 2 hr , but was protected by albumin at 5 mg/ml. The enzyme was more ptable when either total liver or liver homogenate was kept frozen at −20°C. Liver sialidase also retained about 70% of its activity after mechanical homogenization for 5 min. Potential inhibitors, notably, p-aminooxanilic acid, fetuin III, Triton X-100, mucin, sialyllactose, colominic acid, sodium taurocholate, N-acetylneuraminic acid, and methoxyphenyl- N-acetylneuraminic acid, were tested. Sialyllactose, methoxyphenyl- N-acetylneuraminic acid, fetuin, N-acetylneuraminic acid, and colominic acid were competitive inhibitors with K i values of 1.12, 0.37, 0.20, 0.78, and 0.22 m m, respectively. The 0.11 m solutions of NaCl, LiCl, and KCl inhibited 20–30%, and CaCl 2 about 60%, of the enzyme activity.