Abstract
A novel methylotrophic bacterium, strain Zm11T, was isolated from reddish brown snow collected in a moor in Japan. Cells of the isolate were Gram-stain-negative, motile, and rod-shaped (0.6-0.7 × 1.2-2.7μm). Growth was observed at 5-32°C with an optimum growth temperature of 25-28°C. The pH range for growth was 5.4-7.8 with an optimum pH of 6.8. The strain utilized only methanol as carbon and energy sources for aerobic growth. The major cellular fatty acids (> 40% of total) were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16: 0. The predominant quinone was Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The complete genome of strain Zm11T is composed of a circular chromosome (2,800,413bp), with G + C content of 46.4mol%. Phylogenetic analyses were conducted based on the 16S rRNA gene sequence and conserved proteins encoded in the genome. The results of analyses indicate that strain Zm11T is a member of the family Methylophilaceae but does not belong to any existing genus. On the basis of its genomic and phenotypic properties, strain Zm11T (= DSM111909T = NBRC114766T) is proposed as the type strain of a new species in a new genus, Methyloradius palustris gen. nov., sp. nov.
Highlights
Its complete genome sequence is available in the GenBank/EMBL/DDBJ, with accession number of Methylotrophic bacteria can grow on organic single-carbon (C1) compounds as sole sources of energy and carbon
They oxidize methanol to formaldehyde by methanol dehydrogenases (MDHs), which can be classified into two types: MxaFI-type and XoxF-type
A novel methylotrophic bacterium belonging to the family Methylophilaceae was isolated and characterized
Summary
Its complete genome sequence is available in the GenBank/EMBL/DDBJ, with accession number of Methylotrophic bacteria can grow on organic single-carbon (C1) compounds (e.g., methane, methanol and methylated compounds without C-C bond) as sole sources of energy and carbon. The grown culture was transferred to the same medium twice, resulting in enrichment of organisms related to methane-oxidizing bacteria and other methylotrophs. A pure culture of strain Zm11T was obtained by repeated serial dilution in the same medium supplemented 0.1% yeast extract (w/v). Strain Zm11T was grown with DSMZ medium supplemented 1% (v/v) methanol at 28°C.
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