Abstract
An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22T, was isolated from sediment of Hulun Lake, InnerMongolia, China. The organism was found to grow optimally at 30°C in a medium containing 0-0.75% (w/v) NaCl at pH 7.5. The major fatty acid identified was summed feature 8 (C18:1ω7c). The dominant polar lipids were phosphomonoester, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The main respiratory quinone was Q-10. The draft genome sequence of strain L22T consisted of 4354,788bp. The G + C content of genomic DNA was 69.8mol %. The 16S rRNA gene sequences indicated that strain L22T was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae, being most closely related to Methylobrevis pamukkalensis JCM 30229T with 95.9% 16S rRNA gene sequences similarity. The AAI, ANI and dDDH values between strain L22T and M. pamukkalensis JCM 30229T were 72.5%, 80.7% and 22.7%. Based on taxonomic results in this study, we proposed that strain L22T a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae, for which the name Methylobrevis albus sp. nov. is proposed. The type strain is L22T (=KCTC 72858T=MCCC 1H00432T).
Highlights
The genus Methylobrevis was first described by Doronina et al (2015) as a member of the class Alphaproteobacteria within the order Rhizobiales
The 16S rRNA gene sequences indicated that strain L22T was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae, being most closely related to M. pamukkalensis JCM 30229T with 95.9% sequences similarity
Based on taxonomic results in this study, we proposed that strain L22T was placed into a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae, for which the name Methylobrevis albus sp. nov. is proposed
Summary
The genus Methylobrevis was first described by Doronina et al (2015) as a member of the class Alphaproteobacteria within the order Rhizobiales. The genus Methylobrevis is comprised of Gram-stainnegative, strictly aerobic bacteria who do not form endospores or prosthesis. Up to now only one described strain, M. pamukkalensis(Doronina et al 2015), has been confirmed to belong to the genus Methylobrevis within the family Pleomorphomonadaceae, whichwas recently revised with genus Chthonobacter(Kim et al 2017), Hartmannibacter(Suarez et al 2014), Methylobrevis(Doronina et al 2015), Mongoliimonas(Jiao et al 2017), Oharaeibacter(Lv et al 2017) and Pleomorphomonas(Xie and C.H. 2005) supposed as the only six type strains base on LPSN (LPSN, List of Prokaryotic Names with Standing in Nomenclature)(Parte)(Hrdt et al 2020). Genotypic and chemotaxonomic characteristics, the paper aimed to propose a novel species of the genus Methylobrevis within the newly identified family Pleomorphomonadaceae, represented by strain L22T
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