Abstract
Methyl jasmonate is capable of initiating or improving the biosynthesis of secondary metabolites in plants and therefore has opened up a concept for the biosynthesis of valuable constituents. In this study, the effect of different doses of methyl jasmonate (MeJA) elicitation on the accumulation of terpenoid indole alkaloids (TIAs) in the hairy root cultures of the medicinal plant, Rhazya stricta throughout a time course (one-seven days) was investigated. Gas chromatography-mass spectrometry (GC-MS) analyses were carried out for targeted ten major non-polar alkaloids. Furthermore, overall alterations in metabolite contents in elicited and control cultures were investigated applying proton nuclear magnetic resonance (1H NMR) spectroscopy. Methyl jasmonate caused dosage- and time course-dependent significant rise in the accumulation of TIAs as determined by GC-MS. The contents of seven alkaloids including eburenine, quebrachamine, fluorocarpamine, pleiocarpamine, tubotaiwine, tetrahydroalstonine, and ajmalicine increased compared to non-elicited cultures. However, MeJA-elicitation did not induce the accumulation of vincanine, yohimbine (isomer II), and vallesiachotamine. Furthermore, principal component analysis (PCA) of 1H NMR metabolic profiles revealed a discrimination between elicited hairy roots and control cultures with significant increase in total vindoline-type alkaloid content and elevated levels of organic and amino acids. In addition, elicited and control samples had different sugar and fatty acid profiles, suggesting that MeJA also influences the primary metabolism of R. stricta hairy roots. It is evident that methyl jasmonate is applicable for elevating alkaloid accumulation in “hairy root” organ cultures of R. strica.
Highlights
Rhazya stricta is a member of the Apocynaceae family and broadly spread from the Middle East to the northwestern of the Indian sub-continent [1]
The Gas chromatography-mass spectrometry (GC-MS) results revealed that all three concentration levels (50 μM, 100 μM, 200 μM) of methyl jasmonate (MeJA)
In comparison to the control cultures, the content of fluorocarpamine was 3.7-fold increased on the fifth day after cultures were treated with 200 μM MeJA
Summary
Rhazya stricta is a member of the Apocynaceae family and broadly spread from the Middle East to the northwestern of the Indian sub-continent [1]. It is abundantly utilized in traditional medicine for the cure of several illness. R. stricta is a prolific source of terpenoid indole alkaloids (TIAs) and has been extensively investigated since the early 1960s [2]. The total number of isolated and structurally. Because of its wide use in indigenous medicine, the plant has been the focus of a number of pharmacological investigations using in vitro and animal models. Research has disclosed anticancer activities of the crude alkaloid extracts [3] and isolated pure components, e.g., rhazinilam [4], sewarine, and vallesiachotamine [5]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
