Methylglyoxal: Antimicrobial activity against blood culture isolates of Salmonella Typhi and other Gram negative rods.

Raja Kamran Afzal,Syed Azhar Ahmed,Abdul Hannan,Fizza Khalid

doi:10.12669/pjms.35.4.807

Abstract

Objectives:To evaluate the activity of Methylglyoxal against the blood culture isolates of Salmonella Typhi and various Gram negative rods and to compare the activity of Methylglyoxal against S. Typhi and other Gram negative rods.Methods:It was an experimental study conducted at the Department of Microbiology, University of Health Sciences (UHS), Lahore-Pakistan in collaboration with the Department of Microbiology, CMH Lahore, from July 2011 to June 2012. Recent blood culture isolates of S. Typhi and other Gram negative rods were collected from different hospitals of Lahore and kept stored at -80°C. As per the latest CLSI guidelines, morphological, biochemical and serological identification was carried out and antimicrobial susceptibility was tested. A multi-point inoculator was used to carry out agar dilution for determination of MICs of MGO. Results were determined after compilation of data using latest SPSS version.Results:MIC90 of MGO against the clinical isolates of S. Typhi was 0.20 mg/mL (2.8 mM) and against Gram negative rods it was 0.21 mg/mL (3.0 mM). The p-value of MICs of MGO against the isolates of S. Typhi was 0.023 when compared with Gram negative rods (p<0.05; statistically significant).Conclusion:MGO has a scientifically proven in vitro antimicrobial activity against blood culture isolates of S. Typhi and various Gram negative rods.

Highlights

Medical research is continuing towards administration of honey for systemic infections, where conventional therapy is failing
This study aims at evaluating the in vitro antibacterial activity of MGO against the blood culture isolates of S
Typhi and 33 Gram negative rods isolates were tested against a panel of antibiotics

Summary

Introduction

Medical research is continuing towards administration of honey for systemic infections, where conventional therapy is failing. On the basis of its prominent antibacterial activity, honey is being chosen by physicians naturally as an alternate antimicrobial compound.[1] The ‘beeorigin factors of honey are its increased osmolarity, reduced pH and H2O2 released by glucose oxidase. Second is methylglyoxal (MGO), which is the nonperoxide factor of ‘plant-origin’. These are its main contributing antibacterial factors.[2] With the help of chemiluminescence-HPLC, a portion of New Zealand manuka nectar has been isolated which shows antimicrobial activity in a non-peroxide way.[3] As compared to the other flora of honeys, there is atleast 100-folds higher concentration of

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Conclusion