Abstract
Background: Exercise improves function, reduces disability, maintains independence, and improves quality of life for low-grade glioma (LGG) patients. Exercise can also improve the effectiveness of cancer treatment. The goal of this research was to find potential exercise related genes that may be used to predict exercise levels and may be used as a biomarker for cancer outcomes. Methods: The GSE111551 database was thoroughly examined in this research, and the resulting conclusion of exercise-related genes was reached. The protein interaction network (PPI) was used to examine the differentially expressed genes (DEGs). Then the exercise-related gene TLR1 was chosen. The expression, methylation degree, prognosis, and immune relevance of TLR1 were investigated using bioinformatics. In addition, we verified the role of TLR1 in Glioma cell lines. Results: LGG patients with reduced TLR1 expression and hypermethylation had a better overall survival (OS) and progression free survival (PFS), using the TCGA database. Low TLR1 expression and hypermethylation of TLR1 were found to be independent biomarkers for OS using Cox regression. Furthermore, the CGGA database was used to confirm the prognostic function of TLR1 in this cancer. Finally, most methylation sites of TLR1 were strongly correlated with immune infiltration and immune checkpoint. Then, reducing TLR1 expression substantially slowed the cell cycle and decreased LGG cell proliferation, emigration, and infiltration in vitro. Conclusions: Exercise-related gene TLR1 has the potential to be a useful prognostic biomarker, and it is thought to be involved in immune cell infiltration and immunotherapy in LGG.
Highlights
Tumors derived from the neuroepithelium are called gliomas, accounting for 29% of the central nervous system cancers, which is the most common primary intracranial tumor (Du et al, 2021)
Most methylation sites of TLR1 were strongly correlated with immune infiltration and immune checkpoint
Exercise-related gene TLR1 has the potential to be a useful prognostic biomarker, and it is thought to be involved in immune cell infiltration and immunotherapy in low-grade glioma (LGG)
Summary
Tumors derived from the neuroepithelium are called gliomas, accounting for 29% of the central nervous system cancers, which is the most common primary intracranial tumor (Du et al, 2021). Animal experiments and clinical experiments have confirmed the encouraging relationship between appropriate physical exercise and improving the survival results of patients with malignant tumors of the nervous systemanimal (Carson et al, 2007; Wu et al, 2010; Cormie et al, 2015; Tantillo et al, 2020). In other cancer patients and chronic patients, clinical studies have confirmed the efficacy of appropriate exercise in combating physical impairment, cognitive impairment, and psychological effects such as depression and anxiety (Cormie et al, 2015). Reduces disability, maintains independence, and improves quality of life for low-grade glioma (LGG) patients. The goal of this research was to find potential exercise related genes that may be used to predict exercise levels and may be used as a biomarker for cancer outcomes
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