Abstract
Methylation analyses were conducted on 38 NRRL dextrans with the aid of capillary gas-liquid chromatography (g.l.c.)-mass spectrometry. The superior chromatographic resolution of capillary g.l.c. permitted better quantitation of linkage types and detection of trace components than had been obtained with packed columns. Only a few of the dextrans analyzed contain a single type of non-(1→6) linkage; seven contain at least traces of all three possible secondary links at points of branching. The results amend some earlier methylation analyses conducted by packed-column g.l.c., and extend the utility of the NRRL dextran collection by providing more-precise, quantitative data on the types of α- d-glucopyranosyl residues present.
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