Abstract
1527 Background: While patients without malignancy have <100μg/ml free DNA in plasma (Chang, J NC I 2002), patients with malignancy demonstrate substantial quantities of tumor-specific DNA in their plasma. Measurements of this DNA are being studied to develop plasma markers to correlate with tumor burden. This approach has not been explored in gliomas. Methods: This study assesses the presence of plasma tumor-specific DNA in gliomas using methylation specific polymerase chain reaction (MSP). With IRB approval, 30 ml venous blood was collected prior to craniotomy from 10 patients with presumed high grade glioma. After surgery, DNA was extracted and quantified from the tumor and plasma samples. The methylation status of p16, p73, RARβ, and MGMT gene promoters was assayed with MSP. The presence of tumor-specific DNA in the plasma was defined as identification of the same methylated promoter (MP) in both plasma and tumor. Results: Histology was: glioblastoma (N=6), anaplastic astrocytoma (2), anaplastic oligo...
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