Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures.

Thanh-Tam Ho,Hosakatte Niranjana Murthy,So-Young Park

doi:10.3390/ijms21030716

Abstract

Recently, plant secondary metabolites are considered as important sources of pharmaceuticals, food additives, flavours, cosmetics, and other industrial products. The accumulation of secondary metabolites in plant cell and organ cultures often occurs when cultures are subjected to varied kinds of stresses including elicitors or signal molecules. Application of exogenous jasmonic acid (JA) and methyl jasmonate (MJ) is responsible for the induction of reactive oxygen species (ROS) and subsequent defence mechanisms in cultured cells and organs. It is also responsible for the induction of signal transduction, the expression of many defence genes followed by the accumulation of secondary metabolites. In this review, the application of exogenous MJ elicitation strategies on the induction of defence mechanism and secondary metabolite accumulation in cell and organ cultures is introduced and discussed. The information presented here is useful for efficient large-scale production of plant secondary metabolites by the plant cell and organ cultures.

Highlights

In plant defence systems, each cell has acquired the capability to respond to pathogens and environmental stresses and to build up a defence response
The elicitation process leads to crosstalk between jasmonates with their receptors which are present in the plasma membrane, which leads an array of defence responses with the cells, including the production of reactive oxygen species (ROS), reactive nitrogen species (RNS), and induction of enzymes of oxidative stress protection [29]
Majority of the studies have revealed that binding of elicitors to receptors leads to induction of pathogenesis-related proteins and the production of ROS and RNS, enzymes of oxidative stress protection, the activation of defence-related genes [30,31,32,33,34,35]

Summary

Introduction

Each cell has acquired the capability to respond to pathogens and environmental stresses and to build up a defence response. The elicitation process leads to crosstalk between jasmonates with their receptors which are present in the plasma membrane, which leads an array of defence responses with the cells, including the production of reactive oxygen species (ROS), reactive nitrogen species (RNS), and induction of enzymes of oxidative stress protection [29]. This leads to synthesis and accumulation of signaling molecules such as JA, salicylic acid (SA), nitric oxide (NO), ethylene (ET) inside the cell and subsequent regulation of gene expression involved in secondary metabolite production [5,30,31]. Adventitious roots and hairy roots are commonly used as culture system followed by multiple shoots and embryos for elicitation experiments [4,29,32]

Summary of MJ Elicitation Mechanism

Oxidative Stress-Induced by Methyl Jasmonate

Antioxidant Response

Findings

Effect of MJ on Gene Expression and Secondary Metabolite Accumulation