Methyl benzoate and nerolidol attract the cyclocephaline beetle Cyclocephala paraguayensis to trumpet flowers

Abstract

Cyclocephaline beetles are flower visitors attracted primarily by major floral volatiles. Addressing the identity of these volatile compounds is pivotal for understanding the evolution of plant-beetle interactions. We report the identification and field testing of the attractant volatiles from trumpet flowers,Brugmansia suaveolens(Willd.) Sweet (Solanaceae), for the beetleCyclocephala paraguayensisArrow (Melolonthidae: Dynastinae). Analysis of headspace floral volatiles revealed 19 compounds, from which eucalyptol (57%), methyl benzoate (16%), and β-myrcene (6%) were present in the largest amounts, whereas E-nerolidol in much lesser amounts (1.8%). During a first-field assay, traps baited with Mebe alone or blended with the other two major compounds attracted more beetles than myrcene and eucalyptol alone, which did not differ from the negative controls. In a second assay, Mebe and nerolidol attracted more beetles as a blend than individually. Nerolidol was more attractive than Mebe, and all treatments attracted more beetles than negative controls. The number of attracted beetles in the Mebe-nerolidol blend was greater than the combined sum of beetles attracted to these compounds alone, suggesting a synergistic interaction.The attraction ofC. paraguayensisby trumpet-flower volatiles supports the beetle's extended preference for sphingophilous plants, especially when cantharophilous (beetle-pollinated) flowers are lacking. This phenomenon, thus, might have contributed to the widespread occurrence of this beetle throughout the Brazilian biomes.