Methoxyacrylate Fungicide Candidate CL-15C Also Functions as a Plant Elicitor in Arabidopsis thaliana and Oryza sativa L.

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Unlike traditional fungicides targeting fungi, plant elicitors usually lack direct fungicidal activity but improve the plant immune system to resist fungi infection, which has gained increasing attention for better fungi resistance management and environment protection. (E)-methyl-2-(2-((((Z)-(amino-(3,4-dichloroisothiazol-5-yl)methylene)amino)oxy)methyl)phenyl)-2-(methoxyimino)acetate (CL-15C) was found to be a fungicide candidate with a broad spectrum. Here, we studied its immune-inducing ability and mechanism to strengthen the resistance of Arabidopsis thaliana against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and Oryza sativa L. against Magnaporthe oryzae. CL-15C promoted a 2.20- and 1.47-fold increase in phenylalanine ammonia-lyase (PAL) activity in A. thaliana and O. sativa, respectively. It also facilitated a 1.89- and 1.32-fold increase in accumulation of salicylic acid (SA) in A. thaliana and O. sativa, respectively. Differential genes were clustered in the SA signaling pathway at 24 h after a CL-15C treatment in A. thaliana. Because PAL is a rate-limiting enzyme in the phenylalanine metabolic pathway, after a CL-15C treatment, a pal1(PAL 1) mutant was more susceptible to Pst DC3000 when compared with the wild type. Bacterial counts in leaves after a CL-15C treatment showed a 1.11-fold reduction in the pal1 mutant and a 1.54-fold reduction in the wild type. The effect of CL-15C on the PAL enzyme activity and SA content was attenuated in the pal1 mutant. Present experimental data implied that the immune-inducing activity of CL-15C was dependent on PAL gene-mediated synthesis of SA.