Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells.

Abstract

Many studies from different model organisms have demonstrated that microtubules are essential for various cellular processes, including cell division, cell morphogenesis, and intracellular trafficking. In interphase plant cells, oriented cortical microtubule arrays are highly characteristic in cells that display various morphologies, such as elongated hypocotyl cells and root cells, jigsaw-puzzled leaf pavement cells, and petal epidermal conical cells. Conical cells represent a specialized epidermal cell type found in the petal epidermis of many flowering plants. It has been suggested that in the model plant Arabidopsis thaliana, the petal adaxial epidermal cells develop from a roughly hemispherical morphology to a conical shape, correlating with the reorientation of cortical microtubules from random to well-ordered circumferential arrays. This chapter presents an overview of the methods available to visualize the microtubule cytoskeleton in living conical cells via confocal microscopy.