Abstract
This chapter discusses methods that can be used to study oxygen-sensing sodium channels. Various K + and Ca 2+ channels in central neurons and vascular tissue respond to low O 2 levels, and several hypotheses of how this O 2 sensing occurs have been put forward. For example, the channel itself, or an associated subunit, may sense changes in the O 2 levels, there may be a nicotinamide adenine dinucleotide phosphate (NAD(P)H) oxidase associated with the channel in the plasma membrane, or there may be other nonhaem-containing structures closely associated with the channel. Ion channel O 2 sensing involves a redox reaction and/or a haem-containing structure. The chapter describes the dissociation of ventricular myocytes, dissociation of young rat hippocampal neurons, hippocampal cell cultures, and expression of recombinant channels in L9292/HEK293 cell lines. Patch clamping persistent Na + channels, bath and pipette solutions, and the role of INaP in hypoxic cell damage and arrhythmias are discussed as well.
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