Abstract
In the medical field, there are growing interests in applied research such as in vivo fluorescence monitoring because of excellent body transmission characteristic of the near-infrared light. However, optical noise by excitation light and illumination equipment for medical applications such as interior light, surgical light decrease efficiency of the fluorescent signal when observers such as surgeons confirm fluorescence signals in medical field. To solve these problems in medical field, we have analyzed external noise factors by effect on image realization, quantification of optical noise generation by external factors, and have suggested methods of minimize the optical noise in this paper. In case of fluorescence imaging in the operating room, it has been confirmed that fluorescent excitation light, interior light and surgical light are factors to generate optical noise. To acquire near-infrared fluorescence images and to compare fluorescence contrast under conditions of darkroom, interior light and surgical light, light emitting diodes (LEDs) sources that have peak wavelength at 740, 760 and 780 nm respectively were used as excitation light sources. In addition, short-pass filter which has transmission edge at 775 nm has been applied to minimize the optical noise in each external noise factor. By comparing contrast of each image before and after use of the short-pass filter, we confirmed that optical noise reduced 49%, 56% and 66% in external noise factors respectively.
Highlights
Fluorescence imaging technique uses the emission of light by a substance that has absorbed light or other electromagnetic radiation
We placed the short-pass filter in front of excitation light sources (740, 760 and 780 nm) and the interior light and we acquired fluorescence images of the circular gauze coated with Indocyanine green (ICG) solution by using light emitting diodes (LEDs) excitation light sources with center wavelength of 740, 760 and 780 nm under the interior light, respectively
We placed the short-pass filter in front of excitation light sources (740, 760 and 780 nm) and the interior light and we acquired fluorescence images of the circular gauze coated with ICG solution by using LED excitation light sources with center wavelength of 740, 760 and 780 nm under the surgical light, respectively
Summary
Fluorescence imaging technique uses the emission of light by a substance that has absorbed light or other electromagnetic radiation. The emitted light has a longer wavelength, and lower energy, than the absorbed radiation. Near-infrared (NIR) fluorescence monitoring in the range of 700 - 900 nm is being utilized in the bio-medical field actively because NIR has high penetration characteristic in hemoglobin, water and lipid etc. Indocyanine green (ICG) which emits NIR light (center wavelength: 830 nm) has been used in target protein application since ICG was granted to use in clinical research by Food and Drug Administration (FDA) [10]-[14]. ICG is widely used as the synthesized probe and the surgical guide by NIR fluorescence agent and specific substance which is capable of tracking of tumors [17] [18]
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