Abstract
ADP-ribosylation factors (ARF) are GTPases that act to control the activation of numerous signaling events and cellular responses. The ARF6 isoform, present at the plasma membrane, can be activated by the angiotensin II type 1 receptor (AT1R), a process dependent upon β-arrestin recruitment to the activated receptor. Here, we describe classical methods used to assess β-arrestin-dependent activation of ARF6 following agonist stimulation of cells. In addition, because ARF6 and β-arrestin can form a complex, we describe the procedures used to detect the interaction of β-arrestin with this GTPase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
