Methods to detect fungi in seeds of the Austronium graveolens, Bauhinia forficata and Cordia trichotoma (Vell.)

Caliandra Bernardi,Américo Wagner Junior,João Henrique Pietrobom,Maristela Dos Santos Rey

doi:10.33448/rsd-v9i11.9537

Caliandra Bernardi, Américo Wagner Junior + Show 2 more

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https://doi.org/10.33448/rsd-v9i11.9537

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Abstract

The importance of native forest seeds, free of phytopathogens, is a strategy for the implantation of quality forest plantations. The scarcity of information about the health conditions of seeds of native forest species causes the spread of diseases. The objective of this work was to determine a method of sanitary analysis to identify pathogens in seeds of the species of Austronium graveolens, Bauhinia forficata and Cordia trichotoma (Vell.). The treatments consisted of filter paper methods with and without disinfestation in the periods of seven and 15 days, 15 days with water restriction, with BDA medium by seven and 15 days and BDA medium with water restriction during 15 days. For each test, it was used 400 seeds. The results showed that for each forest specie seeds the best detection method was in a Petri dish with culture medium and seed disinfestation, the Austronium graveolens and Bauhinia forficata seeds need an incubation period of 15 days with water restriction and Cordia trichotoma (Vell.) seeds of seven days.

Highlights

The demand for seeds of native species is the result of the need to conserve tropical forests, since they are essential in ecosystem recovery and conservation programs (Carvalho, et al, 2006).Forest species such as the Austronium graveolens, Bauhinia forficata and Cordia trichotoma (Vell.) Arráb. ex Steud are native and they present wide geographical distribution, with various uses, such as medicinal attributes, civil construction, furniture quality and ornamentation (Cassimiro, et al, 2015, Araújo, et al, 2014, Martins, et al, 2013, Silva, et al, 2016).In order to obtain quality seedlings is necessary that the seeds have a good genetic and phytosanitary characteristic
The results showed that for each forest specie seeds the best detection method was in a Petri dish with culture medium and seed disinfestation, the Austronium graveolens and Bauhinia forficata seeds need an incubation period of 15 days with water restriction and Cordia trichotoma (Vell.) seeds of seven days
The fungus present highest incidence in the detection methods tested with the species of Austronium graveolens (Table 2), Bauhinia forficata (Table 3) and Cordia trichotoma (Vell.) (Table 4) was Fusarium sp

Summary

Introduction

The demand for seeds of native species is the result of the need to conserve tropical forests, since they are essential in ecosystem recovery and conservation programs (Carvalho, et al, 2006).Forest species such as the Austronium graveolens, Bauhinia forficata and Cordia trichotoma (Vell.) Arráb. ex Steud are native and they present wide geographical distribution, with various uses, such as medicinal attributes, civil construction, furniture quality and ornamentation (Cassimiro, et al, 2015, Araújo, et al, 2014, Martins, et al, 2013, Silva, et al, 2016).In order to obtain quality seedlings is necessary that the seeds have a good genetic and phytosanitary characteristic. The demand for seeds of native species is the result of the need to conserve tropical forests, since they are essential in ecosystem recovery and conservation programs (Carvalho, et al, 2006). Forest species such as the Austronium graveolens, Bauhinia forficata and Cordia trichotoma (Vell.) Arráb. In order to obtain quality seedlings is necessary that the seeds have a good genetic and phytosanitary characteristic. The genetic factor needs of evaluations to select, but the phytosanitary characteristic is dependent of the cultural practices. The sanitary quality of seeds is one of the most important factors that affect the development of forest crops, due to the microorganisms can cause deterioration in the seeds, as well as subsequent lesions and abnormalities in seedlings, what later difficulty in the implantation of forest stands (Piveta, et al, 2010)

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