Abstract
At present, the most common used method for producing transgenic animal is known as DNA microinjection. It directly injects the exogenous gene into fertilized egg cells by using a syringe with micromanipulation instrument. With the exogenous gene integrated into DNA, a transgenic animal is produced. The founders of this method are Jaenisch, Mintz etc. Gordon and Palmiter [1] successively obtained transgenic animals by this method. This method is commonly used at present, and now the transgenic animal research is mostly going based on the Palmiter method, or the method on its improvement. Wang Minhua [2] reported he produced transgenic rabbits with microinjection method transferring anti plague virus nucleic acid enzyme gene. Application of Krimpenfort produced transgenic bovine using both embryos cultured in vitro and microinjection methods.
Highlights
Transgenic animal is the animal whose genome contains exogenous gene. It adds the exogenous gene into sperm, egg cell or fertilized egg cell through cell fusion, cell reorganization, genetic material transfer, chromosome engineering and gene engineering in according with the pre-design
With the exogenous gene integrated into DNA, a transgenic animal is produced
Gordon and Palmiter [1] successively obtained transgenic animals by this method. This method is commonly used at present, and the transgenic animal research is mostly going based on the Palmiter method, or the method on its improvement
Summary
Transgenic animal is the animal whose genome contains exogenous gene. It adds the exogenous gene into sperm, egg cell or fertilized egg cell through cell fusion, cell reorganization, genetic material transfer, chromosome engineering and gene engineering in according with the pre-design. Pronuclear Microinjection (The Nucleus of Two Fertilized Egg Cells are not Fused) The most common used method for producing transgenic animal is known as DNA microinjection.
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