Methods for the isolation and characterization of constituents of natural products: IV. Amide derivatives of amines with pyruvyl chloride 2,6-dinitrophenylhydrazone

Abstract

Preparation of amide derivatives of primary and secondary aliphatic amines with pyruvyl chloride 2,6-dinitrophenylhydrazone is described. The derivatives form within 5 minutes at room temperature in benzene solution. Primary amine derivatives are yellow, and all members investigated (normal C 1C 18) are solids. The primary amine derivatives have an absorption maximum at 407 mμ in benzene and have a molar absorptivity near 5941. Secondary amine derivatives are orange, and all members investigated (symmetrical, unbranched C 2C 10) are also solids. The secondary amine derivatives have an absorption maximum at 412 mμ in benzene and have a molar absorptivity near 5367. Both classes of amine derivatives have properties which facilitate their isolation directly from lipids.