Abstract
Studies were conducted to determine the suitability of storage below freezing of some antimicrobial effectiveness test inoculum organisms: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Cattdida albicans. Several solutions used for protecting microorganisms subjected to storage below freezing were compared. Comparison of 10% dextrose with other solutions (distilled water; 0.07M phosphate buffer, pH 7.0, with 15% glycerol; and 0.07M phosphate buffer, pH 7.0, with 7.5% dimethyl sulfoxide) demonstrated that inoculum suspensions were most stable when prepared with 10% dextrose. After storage for 6 months at −50° in a freezer, inoculum suspensions prepared with 10% dextrose retained viability and demonstrated suitability for use in antimicrobial effectiveness tests of dosage forms containing preservatives.
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