Abstract
The objective of the present study was to develop a methodology to obtain the enriched fraction of ram seminal vesicle protein 14 (RSVP14). The study was developed using Morada Nova rams, from which semen samples were collected weekly. Seminal plasma proteins were precipitated with cold ethanol, and then 6.15 mg/mL of total proteins were subjected to liquid gelatin affinity chromatography using a Gelatin-Sepharose matrix coupled to an automated chromatographic system. Proteins were eluted into four fractions (A, B, C, [...]
Highlights
The seminal plasma is a complex physiological secretion originated from the testis, epididiymides, and accessory sex glands of the male reproductive tract, playing important roles in sperm capacitation (Plante et al, 2012) and other important events of male reproductive physiology, such as sperm motility and protection, acrosome reaction, fertilization, and initial embryonic development (Moura et al, 2018)
The objective of the present study was to develop a methodology to obtain the enriched fraction of ram seminal vesicle protein 14 (RSVP14)
After separation by heparin affinity chromatography, the non-heparin-binding proteins represented the peak of interest with 30.64% of proteins obtained in the first chromatographic step
Summary
The seminal plasma is a complex physiological secretion originated from the testis, epididiymides, and accessory sex glands of the male reproductive tract, playing important roles in sperm capacitation (Plante et al, 2012) and other important events of male reproductive physiology, such as sperm motility and protection, acrosome reaction, fertilization, and initial embryonic development (Moura et al, 2018). The most abundant proteins in ruminant seminal plasma are members of two families of proteins, binder of sperm proteins (BSP) and spermadhesins. Proteins from BSP family bind to phospholipids and induce the efflux of cholesterol from the spermatic membrane to promote sperm capacitation (Plante et al, 2012). Bergeron et al (2005) indicated that BSP homologues identified in the seminal plasma of rams are defined as ram seminal vesicle proteins (RSVP). According to van Tilburg et al (2013), the main proteins
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