Abstract
Implantation of cannulae into brains of neonatal rats presents methodological difficulties. We discuss such issues as avoiding tissue injury, and describe successful techniques. Cannulae size, methods of preparation, insertion, and securing are evaluated. We present a modified cannula holder applicable to the soft neonatal brain. Application of these methods to the study of glucocorticoid receptors in the neonatal rat hypothalamus is illustrated.
Highlights
Secretion of glucocorticoids (GCs) is stimulated by a variety of stressors acting via corticotropin releasing hormone (CRH) and adrenocorticotropin (ACTH)
In order to study GC negative feedback mechanisms at the hypothalamic level, we developed a technique for chronic implantation of steroids into the paraventricular nucleus (PVN) of neonatal rats
Since some brain growth occurs in a given 72-hr period in neonatal rats, these coordinates aim at the anterior “tip” of the PVN
Summary
Secretion of glucocorticoids (GCs) is stimulated by a variety of stressors acting via corticotropin releasing hormone (CRH) and adrenocorticotropin (ACTH). GCs exert inhibitory feedback effects on the secretion and synthesis of CRH in the hypothalamic paraventricular nucleus (PVN) (Swanson and Simmons, 1989; Dallman et al, 1987; Young et al, 1986) mainly via local GC receptors (Reul and de Kloet, 1985; Kovacs and Mezey, 1987). This regulatory mechanism may not be operative during the first postnatal days in the rat (Grin0 et al, 1989; Baram and Lerner, 1991).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Pharmacological and Toxicological Methods
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
