Abstract
The identification of beta cell and living cells of purified islets of adult rats were comparatively studied with ethanol or DMSO prepared dithizone(DTZ)and with acridine orange (AO)-propidium iodide(PI)or fluorescent diacetate(FDA)-ethidium bromide(EB)respectively.The results indicated that the function and viability of islet cells were not influenced by the stain-ing, the insulin content of the culture medium and the insulin release test of the stained islet were not significantly different from those of the unstained controls(P＞0.05).The cell-toxicity of ethanol prepared DTZ was much less than that of DMSO prepared.In addition, the ethanol pre-pared DTZ was easily preserved.AO-PI staining could identify the apoptotic cells because the binding of AO with double chain DNA showed green fluorescence, while the binding of AO with single chain DNA(degenerated DNA)showed red fluorescence.Therefore, DTZ and AO-PI com-bined staining may exactly identify the purity, amount and viability of the islets. Key words: Islet quality Dithizone Acridine orange Propidium iodide
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