Abstract
Root tissue excised from alfalfa cultivars (Medicago sativa L. and M. media Pers.) was used to determine relationships between impedance, living cell count and duration of low temperature treatment for cold-conditioned material. Correlations exist between all combinations of these three characters. For the cultivars studied, differences were found to exist between regression coefficients for the relationship between tissue impedance and living cell counts when tissue treated with sucrose was compared with tissue receiving no sucrose. The use of sucrose together with cold conditioning was also shown to be effective in detecting small differences in impedance between clones.
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