Abstract
AbstractOn the matter of conservation, pollen is a secure alternative to maintain the variability of date palm (Phoenix dactylifera L.) genetic resources. This chapter describes a protocol developed to cryopreserve pollen from trees in the USDA-ARS National Plant Germplasm System (NPGS). Pollen viability levels were higher prior moisture adjustment content over saturated salts solutions of Ca(NO3)2(46% RH) or MgCl2(33% RH) at 23 °C. After storage in liquid nitrogen, hydration over H2O for 2 h, inoculation on Marquard medium and incubation at 23 °C overnight were identified as optimized in vitro germination conditions. These data suggest that date palm pollen can be successfully stored in genebanks using cryopreservation technique.Key wordsPhoenix dactylifera L. Liquid nitrogen Germination medium Viability assessment
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