Abstract
The ability to measure extremely low concentrations of biologically active molecules in fluid and tissue has enhanced the study of perinatal and neonatal metabolism as it has every other area of biology and medicine. Metabolic processes and biologic regulatory mechanisms are intimately connected, making the measurement of hormones and other biologic agents crucial to the study of metabolism. Even a cursory review of this textbook indicates the key role that such measurements have in a discussion of endocrine, paracrine, and/or autocrine effectors. To measure such effector molecules, both large and small, the technique of immunoassay is most commonly utilized, in which an antibody or group of antibodies can recognize and bind the molecule of interest with high specificity and great sensitivity.
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