Abstract
The purpose of the research is to develop a methodology for the quantitative determination of fenbendazole (FBZ) and its metabolites in organs and tissues of sheep by high performance liquid chromatography tandem mass-spectrometry (HPLC-MS/MS).Materials and methods. The method for determining of FBZ and its metabolites after the administration of the supramolecular complex of fenbendazole was validated in accordance with international guidelines for the following indicators: linearity, recovery, specificity, precision, accuracy, limits of quantitative and qualitative determination.Results and discussion. The method of quantitative analysis of FBZ and its metabolites sulfone and sulfoxide in the body of sheep after the administration of the supramolecular complex has been adapted. The proposed method has a linear relationship (R > 0.99) in the range of 5–1000 ng / g and has shown a good reproducibility and accuracy.
Highlights
Для повышения растворимости, биодоступности и эффективности препарата была применена механохимическая технология с использованием поливинилпирролидона для адресной доставки, что позволило значительно повысить антигельминтную активность и снизить в 2–3 раза терапевтическую дозу
The purpose of the research is to develop a methodology for the quantitative determination of fenbendazole (FBZ) and its metabolites in organs and tissues of sheep by high performance liquid chromatography tandem mass-spectrometry (HPLC-MS/MS)
The method for determining of FBZ and its metabolites after the administration of the supramolecular complex of fenbendazole was validated in accordance with international guidelines for the following indicators: linearity, recovery, specificity, precision, accuracy, limits of quantitative and qualitative determination
Summary
А. Методика количественного определения фенбендазола и его метаболитов в органах и тканях животных методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием // Российский паразитологический журнал. Контент доступен под лицензией Creative Commons Attribution 4.0 License. The content is available under Creative Commons Attribution 4.0 License. Russian Journal of Parasitology / Российский паразитологический журнал 2021;15(4):
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