Abstract
The monosaccharide composition of four different samples of brown seaweeds Laminaria digitata and Saccharina latissima were compared by different high performance anion exchange chromatography (HPAEC) methods after different acid hydrolysis treatments or a cellulase treatment.
Highlights
Carbohydrates from brown macroalgae have received increased attention, in Europe, as a new biomass resource for biofuels and manufacture of highvalue carbohydrate products.[1,2] the proper assessment of the potential of this new resource for biore nery purposes requires fast and reliable characterization of the biomass, notably with respect to the carbohydrate composition.Several extraction and determination methods for particular compounds have been developed but no methods exist for total quanti cation of the carbohydrate contents and carbohydrate composition of brown seaweeds.The composition of polysaccharides in terrestrial plant materials is usually determined by measuring the monosaccharide release a er acid hydrolysis
The primary objective of this study was to examine the in uence of different biomass material hydrolysis treatments and compare different high performance chromatography carbohydrate determination methods (borate vs. alkaline (NaOH) elution) in order to identify an optimal strategy for determination of all structural carbohydrate monomers from one hydrolysate of brown seaweed
Determination of the fucose levels was less affected by the type of acid treatment, but as expected, the fucose levels tended to be higher in the samples harvested in the spring than in August (L. digitata Apr'[12] vs. Aug'[12], Table 2)
Summary
Several extraction and determination methods for particular compounds have been developed but no methods exist for total quanti cation of the carbohydrate contents and carbohydrate composition of brown seaweeds. The composition of polysaccharides in ( brous) terrestrial plant materials is usually determined by measuring the monosaccharide release a er acid hydrolysis. The optimal type of acid hydrolysis treatment depends on the type of plant material, and no universal method exists. Rich in uronic acids, treatment with hydrochloric acid (HCl) or tri uoroacetic acid (TFA) is usually favored,[3,4] whereas for lignocellulosic biomass acid hydrolysis with sulfuric acid (H2SO4) is generally the norm.[5,6,7] Analogously, different chromatography quanti cation techniques have subsequently been employed to assess the composition of the constituent monosaccharides
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
